Immunology laboratory
Based on xMAP technology for the simultaneous detection of the more than 500 markers in suspension or on solid support, accompanied by ELISA, CLIA, and nephelometric techniques
Allow simultaneous determination of the qualitative and quantitative aspects of a large number of biomarkers, intracellular signals, cytokines, chemokines, and growth factors, metabolic markers, nuclear receptors and transcription factors, some of them for routine clinical use, others for use in research.
The laboratory include the following devices:
- xMAP 3D technology and Microarray (on microchips)
- automatic analyzers for determination with the aid of a series of techniques: ELISA, CLIA, ECLIA, nephelometry, turbidimetry, spectrophotometry, coagulometry and aggregometry
- complete line of electrophoresis / blotting, automatic electrophoretic system for protein separation and identification.
Cytology Laboratory
Allows, in selected cases, the histochemical analysis of tissue samples. Biopsy samples will also be investigated here, the infrastructure of the laboratory also allowing tissue component separation by laser microdissection techniques.
The laboratory include the following devices:
- 4-laser flow cytometer
- LASER microdissection system
- devices for cell separation/ culture/ genetic modification/ analysis
Chromatography and mass spectrometry laboratory
The lab is equipped with LC-MS/MS QTOF triple quadrupole time of flight mass spectrometer.
QTOF detection in LC-MS/MS technique gained great popularity in biomedical research laboratories across Europe, due to its outstanding performance, being present in Romania only in few research centers. The model in the CCAMF was the first of its kind in Romania.
Molecular Biology / Genetics Laboratory
The Next generation sequencing technology will enable sequencing of the entire genome and/or exons in order to identify novel mutations involved in the development of some types of diseases. Patterns of genetic risk will be established through multiple approaches:
- genetic linkage studies
- genetic association studies
- genome-wide association studies (GWAS)
- a new direction is the study of non-coding RNAs.
Further substudies will be added to these in order to explore rare diseases – Pathogenetic mechanisms of consciousness disorders.
The laboratory is equipped with the latest technology:
- automatic nucleic acid separator
- traditional PCR and real-time qPCR systems
- capillary electrophoresis sequencing system with 24 capillaries, with diagnostic ability d. next generation sequencing system
- microarray gene sequencing system
- analysis of amplification products by electrophoresis
Microscopy laboratory
Include the following devices:
- state-of-the-art confocal microscope
- two fluorescence microscopes